PLK4 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PLK4 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28750-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 28750-1-AP (same clone as 28750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 28750-1-AP (same clone as 28750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 28750-1-AP (same clone as 28750-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using PLK4 antibody (28750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

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Product Information

28750-1-PBS targets PLK4 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLK4 fusion protein Ag30482
• Full Name: polo-like kinase 4 (Drosophila)
• Calculated Molecular Weight: 970 aa, 109 kDa
• GenBank Accession Number: BC036023
• Gene Symbol: PLK4
• Gene ID (NCBI): 10733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.