PLK4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLK4 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 28750-1-AP

Synonyms

PLK 4, PLK4, Polo like kinase 4, SAK, STK18

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Validation Data Gallery

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  IHC staining of human colon cancer using 28750-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28750-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28750-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28750-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE1 using 28750-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using PLK4 antibody (28750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

28750-1-AP targets PLK4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLK4 fusion protein Ag30482
• Full Name: polo-like kinase 4 (Drosophila)
• Calculated Molecular Weight: 970 aa, 109 kDa
• GenBank Accession Number: BC036023
• Gene Symbol: PLK4
• Gene ID (NCBI): 10733
• RRID: AB_2881206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Advanced glycation end products initiate the mutual promoting cycle between centrosome amplification and the release of inflammatory cytokines in human vascular endothelial cells; Authors - Jun Zhang ; KD Validated
human	IF	Cell; Time-series reconstruction of the molecular architecture of human centriole assembly; Authors - Marine H Laporte

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Franziska (Verified Customer) (02-15-2023)

RPE1 WT cells were starved for 2 days. Fixed with -20°C Ethanol for 10 min at room temperature (RT), permeablized with 0.3% PBST(triton), blocked with 1%BSA in PBS for 1h at RT, PLK4 (rb, 1:50) and ARL13B (ms, 1:200) in 1% BSA in PBS diluted, prim AK Incubation for 1h at RT. Sek Ak goat anti rb (green) and goat anti mouse (pink). PLK4 can be found at the Basal Body of cilia and also stains cytoskeleton. PLK4 antibody could be tried with 1:200 dilution, to reduce background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50, maybe 1:200 better
• Cell Tissue Type: hTERT-RPE1

PLK4 Antibody Immunofluorescence validation (1:50, maybe 1:200 better dilution) in hTERT-RPE1 (Cat no:28750-1-AP)