CoraLite® Plus 488-conjugated PLK2 Recombinant antibody

PLK2 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230520B2

Cat no : CL488-83431

Synonyms

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Validation Data Gallery

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  IF Staining of hTERT-RPE1 using CL488-83431

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 PLK2 antibody (CL488-83431, Clone: 230520B2 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83431 is PLK2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PLK2 fusion protein Ag29837
• Full Name: polo-like kinase 2 (Drosophila)
• Calculated Molecular Weight: 685 aa, 78 kDa
• GenBank Accession Number: BC013879
• Gene Symbol: PLK2
• Gene ID (NCBI): 10769
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).