PLEC Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis of A431 using 83898-1-RR (same clone as 83898-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83898-1-PBS in a different storage buffer formulation.

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  WB analysis using 83898-1-RR (same clone as 83898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83898-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00839-4

  Sandwich ELISA standard curve of MP00839-4, Human PLEC/Plectin Recombinant Matched Antibody Pair - PBS only. 83898-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29418. 83898-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

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  Sample test of MP00839-4

  U2OS cell lysate, NIH/3T3 cell lysate and C6 cell lysate were measured. The human PLEC/Plectin concentration was determined to be 212.14 ng/mg total protein in U2OS cell lysate, 3,895.97 ng/mg total protein in NIH/3T3 cell lysate and 609.19 ng/mg total protein in C6 cell lysate.

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  IF Staining of A431 using 83898-1-RR (same clone as 83898-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLEC antibody (83898-1-RR, Clone: 240891A10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83898-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00839-2

  Cytometric bead array standard curve of MP00839-2, PLEC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83898-1-PBS. Detection antibody: 83898-2-PBS. Standard: Ag29418. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP00839-3

  Cytometric bead array standard curve of MP00839-3, PLEC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83898-1-PBS. Detection antibody: 83898-3-PBS. Standard: Ag29418. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83898-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83898-1-PBS against Human PLEC was performed. The affinity constant is 0.164 nM.

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  Affinity and Kinetic Characterization of 83898-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83898-1-RR against Human PLEC were performed. The affinity constant is 52.7 pM.

PLEC {ptg:Brand} Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83898-1-PBS

Clone No.240891A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

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Product Information

83898-1-PBS targets PLEC as part of a matched antibody pair:

MP00839-2: 83898-1-PBS capture and 83898-2-PBS detection (validated in Cytometric bead array)

MP00839-3: 83898-1-PBS capture and 83898-3-PBS detection (validated in Cytometric bead array)

MP00839-4: 83898-4-PBS capture and 83898-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PLEC fusion protein Ag29418
• Full Name: plectin 1, intermediate filament binding protein 500kDa
• Calculated Molecular Weight: 532 kDa
• Observed Molecular Weight: 510 kDa
• GenBank Accession Number: NM_201380
• Gene Symbol: Plectin
• Gene ID (NCBI): 5339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15149
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)