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  • Western Blot (WB) analysis of A431 cells using PLEC Recombinant antibody (83898-1-RR)

    WB analysis of A431 using 83898-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLEC Recombinant antibody (83898-1-RR)

    WB analysis using 83898-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PLEC Recombinant antibody (83898-1-RR)

    IF Staining of A431 using 83898-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLEC antibody (83898-1-RR, Clone: 240891A10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83898-1-RR

    Affinity and Kinetic Characterization of 83898-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83898-1-RR against Human PLEC were performed. The affinity constant is 52.7 pM.

PLEC Recombinant antibody

PLEC Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83898-1-RR
Clone No.240891A10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHaCaT cells, A431 cells, HEK-293 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83898-1-RR targets PLEC in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLEC fusion protein Ag29418 Predict reactive species
Full Name plectin 1, intermediate filament binding protein 500kDa
Calculated Molecular Weight 532 kDa
Observed Molecular Weight 510 kDa
GenBank Accession NumberNM_201380
Gene Symbol Plectin
Gene ID (NCBI) 5339
RRIDAB_3671479
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15149
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

Protocols

Product Specific Protocols
WB protocol for PLEC antibody 83898-1-RRDownload protocol
IF protocol for PLEC antibody 83898-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols