PLAP Monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

  WB result of PLAP antibody (60294-1-Ig, 1:1000) with si-control and si-PLAP transfected HepG2 cells. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60294-1-Ig (same clone as 60294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PLAP Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60294-1-PBS

Clone No.2B7C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

PLAP-1, PALP, EC:3.1.3.1, ALPP, ALP

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Product Information

60294-1-PBS targets PLAP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13361

  Product name: Recombinant human ALPP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 199-535 aa of BC009647

  Sequence: SARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDAAHPGRSVVPALLPLLAGTLLLLETATAP

• Full Name: alkaline phosphatase, placental (Regan isozyme)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC009647
• Gene Symbol: PLAP
• Gene ID (NCBI): 250
• RRID: AB_2881409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P05187
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.