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PLAP Monoclonal antibody
PLAP Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2B7C9
Cat no : 60294-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | SCaBER cells, HepG2 cells, human placenta tissue, HT-1376 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
60294-1-Ig targets PLAP in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PLAP fusion protein Ag13361 |
| Full Name | alkaline phosphatase, placental (Regan isozyme) |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC009647 |
| Gene Symbol | PLAP |
| Gene ID (NCBI) | 250 |
| RRID | AB_2881409 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLAP antibody 60294-1-Ig | Download protocol |
| IHC protocol for PLAP antibody 60294-1-Ig | Download protocol |
| FC protocol for PLAP antibody 60294-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs. | ||
Front Bioeng Biotechnol Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects | ||
Biomed Pharmacother The multi-protective effect of IL-37-Smad3 against ox-LDL induced dysfunction of endothelial cells | ||
J Biomed Mater Res A Copper ions-photo dual-crosslinked alginate hydrogel for angiogenesis and osteogenesis | ||
