PLAA Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PLAA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C10G9

Cat no : 60244-1-PBS

Synonyms

DOA1, PLA2P, PLAA, PLAP

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 60244-1-Ig (same clone as 60244-1-PBS)

  WB result of PLAA antibody (60244-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 60244-1-Ig (same clone as 60244-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 60244-1-Ig (same clone as 60244-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 60244-1-Ig (same clone as 60244-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 60244-1-Ig (same clone as 60244-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60244-1-Ig (same clone as 60244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60244-1-Ig (same clone as 60244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

---

Product Information

60244-1-PBS targets PLAA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLAA fusion protein Ag3224
• Full Name: phospholipase A2-activating protein
• Calculated Molecular Weight: 738 aa, 81 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC032551
• Gene Symbol: PLAA
• Gene ID (NCBI): 9373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.