PLAA Monoclonal antibody

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• KD/KO Validated

PLAA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5C10G9

Cat no : 60244-1-Ig

Synonyms

DOA1, PLA2P, PLAA, PLAP

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Validation Data Gallery

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  WB analysis of MCF-7 using 60244-1-Ig

  WB result of PLAA antibody (60244-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells.

• 

  WB analysis of ROS1728 using 60244-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60244-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60244-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 60244-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60244-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 60244-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: ROS1728 cells, K-562 cells, MCF-7 cells, A431 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60244-1-Ig is PLAA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLAA fusion protein Ag3224
• Full Name: phospholipase A2-activating protein
• Calculated Molecular Weight: 738 aa, 81 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC032551
• Gene Symbol: PLAA
• Gene ID (NCBI): 9373
• RRID: AB_2881366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.