CoraLite® Plus 488-conjugated PKIA Polyclonal antibody

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  IF Staining of HeLa using CL488-11743

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PKIA antibody (CL488-11743) at dilution of 1:200.

PKIA Polyclonal Antibody for IF/ICC

Cat No. CL488-11743

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

PKI alpha, PKIA, PRKACN1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11743 targets PKIA in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKIA fusion protein Ag2338
• Full Name: protein kinase (cAMP-dependent, catalytic) inhibitor alpha
• Calculated Molecular Weight: 76 aa, 8 kDa
• GenBank Accession Number: BC022265
• Gene Symbol: PKIA
• Gene ID (NCBI): 5569
• RRID: AB_3672511
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen Affinity Purified
• UNIPROT ID: P61925
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

cAMP-dependent protein kinase inhibitor alpha (PKIA) is also named as PRKACN1, and belongs to the PKI family. Among them, PKIA, a member of protein kinase A (PKA) inhibitor family, was found to be most significantly and highly expressed in susceptible animals (PMID:24910983). Decreased PKIA signaling would directly impact PKA activation, potentially inducing DRP1 phosphorylation and increasing ER Ca2+ stores (PMID:32152556). miR-129-5p activated PKA to regulate the phosphorylation of beta-catenin and cAMP-response element binding protein (CREB) by inhibiting PKIA (PMID:36222334).