CoraLite®594-conjugated PKC Alpha Monoclonal antibody

PKC Alpha Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D11F4

Cat no : CL594-66421

Synonyms

AAG6, PKC A, PKC alpha, PKCA, PKCα, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66421

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 PKC Alpha antibody (CL594-66421, Clone: 1D11F4 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66421 targets PKC Alpha in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PKC Alpha fusion protein Ag17275
• Full Name: protein kinase C, alpha
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC109274
• Gene Symbol: PKC Alpha
• Gene ID (NCBI): 5578
• RRID: AB_2883580
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.