PIP5K1A Monoclonal antibody, PBS Only

PIP5K1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E5G12

Cat no : 67483-1-PBS

Synonyms

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

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Validation Data Gallery

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  WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

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Product Information

67483-1-PBS targets PIP5K1A in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PIP5K1A fusion protein Ag8355
• Full Name: phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
• Calculated Molecular Weight: 56 kDa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC007833
• Gene Symbol: PIP5K1A
• Gene ID (NCBI): 8394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.