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PIP5K1A Monoclonal antibody

PIP5K1A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E5G12

Cat no : 67483-1-Ig

Synonyms

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PIP5K1A Monoclonal antibody (67483-1-Ig)

    IHC staining of human tonsillitis using 67483-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PIP5K1A Monoclonal antibody (67483-1-Ig)

    IHC staining of human tonsillitis using 67483-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, Hela cell, HepG2 cells, L02 cells, K-562 cells, Jurkat cells, MOLT-4 cells, HeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67483-1-Ig targets PIP5K1A in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PIP5K1A fusion protein Ag8355
Full Name phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Calculated Molecular Weight 56 kDa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC007833
Gene Symbol PIP5K1A
Gene ID (NCBI) 8394
RRIDAB_2882710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

Protocols

Product Specific Protocols
WB protocol for PIP5K1A antibody 67483-1-IgDownload protocol
IHC protocol for PIP5K1A antibody 67483-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols