PI3 Kinase p85 Beta Recombinant antibody

PI3 Kinase p85 Beta Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240531B5

Cat no : 83606-5-RR

Synonyms

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

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Validation Data Gallery

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  WB analysis using 83606-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83606-5-RR (PIK3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 83606-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 83606-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
• Positive IF/ICC detected in: Jurkat cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83606-5-RR is PI3 Kinase p85 Beta Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PI3 Kinase p85 Beta fusion protein Ag14367
• Full Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Calculated Molecular Weight: 728 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC014170
• Gene Symbol: PI3 Kinase p85 Beta
• Gene ID (NCBI): 5296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).