PI3 Kinase p85 Beta Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83606-5-RR (same clone as 83606-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83606-5-RR (PIK3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 83606-5-RR (same clone as 83606-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83606-5-RR (same clone as 83606-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

PI3 Kinase p85 Beta Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83606-5-PBS

Clone No.240531B5

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

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Product Information

83606-5-PBS targets PI3 Kinase p85 Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14367

  Product name: Recombinant human PIK3R2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-324 aa of BC014170

  Sequence: YRHESLAQYNAKLDTRLLYPVSKYQQDQIVKEDSVEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQGQTQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQQLRAQASDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGARQKKINEWLGIKNETEDQYALMEDEDDLPHHEERTWYVGKINRTQAEEMLSGKRDGTFLIRESSQRGCYACSVVVDGDTKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHASLVQHNDALTVTLAHPVRAPGPGPPPAAR

• Full Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Calculated Molecular Weight: 728 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC014170
• Gene Symbol: PI3 Kinase p85 Beta
• Gene ID (NCBI): 5296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O00459
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).