PI3 Kinase p85 Alpha Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PI3 Kinase p85 Alpha Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G3C11

Cat no : 60225-1-PBS

Synonyms

P85a; PIK3R1, PI3K p85 alpha, PI3K p85 α, PIK3R1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

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Validation Data Gallery

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  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60225-1-Ig (same clone as 60225-1-PBS)

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60225-1-Ig (same clone as 60225-1-PBS)

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 60225-1-Ig (same clone as 60225-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 60225-1-Ig (same clone as 60225-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 60225-1-Ig (same clone as 60225-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 60225-1-Ig (same clone as 60225-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60225-1-Ig (same clone as 60225-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 60225-1-Ig (same clone as 60225-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 60225-1-Ig (same clone as 60225-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 60225-1-Ig (same clone as 60225-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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  IP experiment of RAW 264.7 using 60225-1-Ig (same clone as 60225-1-PBS)

  IP result of anti-PI3 Kinase p85 Alpha (IP:60225-1-Ig, 5ug; Detection:60225-1-Ig 1:500) with RAW 264.7 cells lysate 1480ug. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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Product Information

60225-1-PBS targets PI3 Kinase p85 Alpha in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PI3 Kinase p85 Alpha fusion protein Ag2344
• Full Name: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC030815
• Gene Symbol: PI3 Kinase p85 Alpha
• Gene ID (NCBI): 5295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.