PI4KA Recombinant antibody

PI4KA Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230057G2

Cat no : 82938-1-RR

Synonyms

PI4-kinase alpha, PI4K-alpha, Phosphatidylinositol 4-Kinase III alpha, Phosphatidylinositol 4-kinase alpha, EC:2.7.1.67

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Validation Data Gallery

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  WB analysis using 82938-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82938-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 82938-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82938-1-RR (PI4KA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 82938-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of MCF-7 using 82938-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PI4KA Recombinant antibody (82938-1-RR, Clone:230057G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82938-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82938-1-RR against Human PI4KA were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, MCF-7 cells, HEK-293T cells, THP-1 cells, SKOV-3 cells, rat brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82938-1-RR targets PI4KA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PI4KA fusion protein Ag3062
• Full Name: phosphatidylinositol 4-kinase, catalytic, alpha
• Calculated Molecular Weight: 2044 aa, 231 kDa
• Observed Molecular Weight: 231 kDa
• GenBank Accession Number: BC018120
• Gene Symbol: PI4KA
• Gene ID (NCBI): 5297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).