PI4KA Recombinant antibody, PBS Only

PI4KA Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230057G2

Cat no : 82938-1-PBS

Synonyms

FLJ16556, PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha

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Validation Data Gallery

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  WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82938-1-PBS targets PI4KA in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PI4KA fusion protein Ag3062
• Full Name: phosphatidylinositol 4-kinase, catalytic, alpha
• Calculated Molecular Weight: 2044 aa, 231 kDa
• Observed Molecular Weight: 231 kDa
• GenBank Accession Number: BC018120
• Gene Symbol: PI4KA
• Gene ID (NCBI): 5297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).