PI3 Kinase p55 Gamma Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PI3 Kinase p55 Gamma Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27035-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of MCF-7 using 27035-1-AP (same clone as 27035-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27035-1-AP (PI 3 Kinase p55 gamma antibody at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 27035-1-AP (same clone as 27035-1-PBS)

  WB result of PI 3 Kinase p55 gamma antibody (27035-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI 3 Kinase p55 gamma transfected HEK-293 cells. This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 27035-1-AP (same clone as 27035-1-PBS)

  WB result of PIK3R3 antibody (27035-1-AP) on whole cell extract from HUVEC targeted by shRNAs (#1, #2) or a control shRNA (NT). (Data from Dr. Fuqiang Geng, Weill Cornell Medical College). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 27035-1-AP (same clone as 27035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 27035-1-AP (same clone as 27035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

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Product Information

27035-1-PBS targets PI3 Kinase p55 Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI3 Kinase p55 Gamma fusion protein Ag25884
• Full Name: phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC021622
• Gene Symbol: PI3 Kinase p55 Gamma
• Gene ID (NCBI): 8503
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.