PI3 Kinase p55 Gamma Polyclonal antibody

• Featured Product
• KD/KO Validated

PI3 Kinase p55 Gamma Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27035-1-AP

Synonyms

p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3

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Validation Data Gallery

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  WB analysis of MCF-7 using 27035-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27035-1-AP (PI 3 Kinase p55 gamma antibody at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293 using 27035-1-AP

  WB result of PI 3 Kinase p55 gamma antibody (27035-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI 3 Kinase p55 gamma transfected HEK-293 cells.

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  WB analysis of HUVEC using 27035-1-AP

  WB result of PIK3R3 antibody (27035-1-AP) on whole cell extract from HUVEC targeted by shRNAs (#1, #2) or a control shRNA (NT). (Data from Dr. Fuqiang Geng, Weill Cornell Medical College).

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  IHC staining of human kidney using 27035-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 27035-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 27035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HUVEC cells, HEK-293 cells
• Positive IHC detected in: human kidney tissue, mouse testis tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

27035-1-AP targets PI3 Kinase p55 Gamma in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI3 Kinase p55 Gamma fusion protein Ag25884
• Full Name: phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC021622
• Gene Symbol: PI3 Kinase p55 Gamma
• Gene ID (NCBI): 8503
• RRID: AB_2880730
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.

Publications

Species	Application	Title
human	IHC	Am J Physiol Lung Cell Mol Physiol; p55PIK Deficiency and its N-terminal Derivative inhibit Inflammation and Emphysema in COPD mouse model.; Authors - Ting Wang
human,mouse	WB,IHC	BMC Cancer; Circ_0021350 plays an oncogene role by regulating miR-1207-3p/PIK3R3 in glioblastoma; Authors - Cheng Tan ; KD Validated
rat	WB	World J Mens Health; Relaxin-2 Prevents Erectile Dysfunction by Cavernous Nerve, Endothelial and Histopathological Protection Effects in Rats with Bilateral Cavernous Nerve Injury; Authors - Kang Liu