PHLPP Monoclonal antibody, PBS Only

• 

  WB analysis of mouse brain using 67640-1-Ig (same clone as 67640-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67640-1-Ig (same clone as 67640-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67640-1-Ig (same clone as 67640-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67640-1-Ig (same clone as 67640-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

PHLPP Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67640-1-PBS

Clone No.2E8E6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse , rat, pig

Applications

WB, Indirect ELISA

Synonyms

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP

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Product Information

67640-1-PBS targets PHLPP in WB, Indirect ELISA applications and shows reactivity with Human, mouse , rat, pig samples.

• Tested Reactivity: Human, mouse , rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PHLPP fusion protein Ag18982
• Full Name: PH domain and leucine rich repeat protein phosphatase
• Calculated Molecular Weight: 1717 aa, 184 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC126277
• Gene Symbol: PHLPP
• Gene ID (NCBI): 23239
• RRID: AB_2882840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60346
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.