PHLPP Monoclonal antibody

PHLPP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse , rat, pig and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2E8E6

Cat no : 67640-1-Ig

Synonyms

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP

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Validation Data Gallery

• 

  WB analysis of mouse brain using 67640-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67640-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67640-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67640-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, pig brain tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

67640-1-Ig targets PHLPP in WB, ELISA applications and shows reactivity with Human, mouse , rat, pig samples.

• Tested Reactivity: Human, mouse , rat, pig
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PHLPP fusion protein Ag18982
• Full Name: PH domain and leucine rich repeat protein phosphatase
• Calculated Molecular Weight: 1717 aa, 184 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC126277
• Gene Symbol: PHLPP
• Gene ID (NCBI): 23239
• RRID: AB_2882840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway.; Authors - Zong-Ze Zhang
pig	WB	Pediatr Res; Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets; Authors - Agus Suryawan
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu