PHKG2 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 83663-2-RR (same clone as 83663-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83663-2-RR (PHKG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83663-2-RR (same clone as 83663-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83663-2-RR (PHKG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83663-2-RR (same clone as 83663-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83663-2-RR (PHKG2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83663-2-RR (same clone as 83663-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (83663-2-RR, Clone: 240643E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 83663-2-RR (same clone as 83663-2-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug PHKG2 Recombinant antibody (83663-2-RR, Clone:240643E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83663-2-RR (same clone as 83663-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PHKG2 Recombinant antibody (83663-2-RR, Clone:240643E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00632-2

  Cytometric bead array standard curve of MP00632-2, PHKG2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83663-4-PBS. Detection antibody: 83663-2-PBS. Standard: Ag6829. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83663-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83663-2-RR against Human PHKG2 were performed. The affinity constant is 1.90 nM.

PHKG2 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83663-2-PBS

Clone No.240643E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

PHK-gamma-T, PHK-gamma-LT, PHK gamma T, GSD9C, EC:2.7.11.19

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Product Information

83663-2-PBS targets PHKG2 as part of a matched antibody pair:

MP00632-2: 83663-4-PBS capture and 83663-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PHKG2 fusion protein Ag6829
• Full Name: phosphorylase kinase, gamma 2 (testis)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002541
• Gene Symbol: PHKG2
• Gene ID (NCBI): 5261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15735
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa.