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PHKG2 Recombinant antibody

PHKG2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240643E1

Cat no : 83663-2-RR

Synonyms

PHK-gamma-T, PHK-gamma-LT, PHK gamma T, GSD9C, EC:2.7.11.19

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHKG2 Recombinant antibody (83663-2-RR)

    WB analysis using 83663-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83663-2-RR (PHKG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PHKG2 Recombinant antibody (83663-2-RR)

    IF Staining of HEK-293 using 83663-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (83663-2-RR, Clone: 240643E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HEK-293T cells using PHKG2 Recombinant antibody (83663-2-RR)

    FC experiment of HEK-293T using 83663-2-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug PHKG2 Recombinant antibody (83663-2-RR, Clone:240643E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using PHKG2 Recombinant antibody (83663-2-RR)

    FC experiment of HepG2 using 83663-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PHKG2 Recombinant antibody (83663-2-RR, Clone:240643E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83663-2-RR

    Affinity and Kinetic Characterization of 83663-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83663-2-RR against Human PHKG2 were performed. The affinity constant is 1.90 nM.

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, mouse liver tissue
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293T cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83663-2-RR is PHKG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PHKG2 fusion protein Ag6829
Full Name phosphorylase kinase, gamma 2 (testis)
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC002541
Gene Symbol PHKG2
Gene ID (NCBI) 5261
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa.

Protocols

Product Specific Protocols
WB protocol for PHKG2 antibody 83663-2-RRDownload protocol
IF protocol for PHKG2 antibody 83663-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols