PHKG2 Polyclonal antibody, PBS Only

PHKG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15109-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 15109-1-AP (same clone as 15109-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 antibody) at dilution of 1:300. This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15109-1-AP (same clone as 15109-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15109-1-AP (same clone as 15109-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15109-1-AP (same clone as 15109-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

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Product Information

15109-1-PBS targets PHKG2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHKG2 fusion protein Ag6829
• Full Name: phosphorylase kinase, gamma 2 (testis)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002541
• Gene Symbol: PHKG2
• Gene ID (NCBI): 5261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa.