PHKG2 Polyclonal antibody

PHKG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15109-1-AP

Synonyms

Phosphorylase b kinase gamma catalytic chain, liver/testis isoform, PHK-gamma-T, PHK-gamma-LT, PHK gamma T, GSD9C

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Validation Data Gallery

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  WB analysis of HEK-293 using 15109-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15109-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15109-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 antibody) at dilution of 1:300.

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  IHC staining of human liver cancer using 15109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 15109-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 15109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15109-1-AP targets PHKG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHKG2 fusion protein Ag6829
• Full Name: phosphorylase kinase, gamma 2 (testis)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002541
• Gene Symbol: PHKG2
• Gene ID (NCBI): 5261
• RRID: AB_2165529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Lactate metabolism is essential in early-onset mitochondrial myopathy; Authors - Zhenkang Chen