PHGDH Recombinant antibody, PBS Only

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  WB analysis of HEK-293 using 81986-1-RR (same clone as 81986-1-PBS)

  WB result of PHGDH antibody (81986-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHGDH transfected HEK-293 cells. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

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  WB analysis using 81986-1-RR (same clone as 81986-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81986-1-RR (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81986-1-RR (same clone as 81986-1-PBS)

  IP result of anti-PHGDH (IP:81986-1-RR, 4ug; Detection:81986-1-RR 1:10000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81986-1-RR (same clone as 81986-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81986-1-RR (PHGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81986-1-RR (same clone as 81986-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81986-1-RR (PHGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81986-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81986-1-RR against Human PHGDH were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

PHGDH {ptg:Brand} Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 81986-1-PBS

Clone No.5L16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 5L16, 3-PGDH, 3PGDH

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Product Information

81986-1-PBS targets PHGDH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PHGDH fusion protein Ag6445
• Full Name: phosphoglycerate dehydrogenase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC000303
• Gene Symbol: PHGDH
• Gene ID (NCBI): 26227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43175
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).