PGM5 Recombinant antibody, PBS Only

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  WB analysis using 82965-1-RR (same clone as 82965-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82965-1-RR (PGM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82965-1-RR (same clone as 82965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82965-1-RR (same clone as 82965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 82965-1-RR (same clone as 82965-1-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human PGM5 (82965-1-RR, Clone:230206C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82965-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82965-1-RR against Human PGM5 were performed. The affinity constant is 0.719 nM.

PGM5 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 82965-1-PBS

Clone No.230206C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

PGMRP, Aciculin, 230206C7

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Product Information

82965-1-PBS targets PGM5 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PGM5 fusion protein Ag34399
• Full Name: phosphoglucomutase 5
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: NM_021965
• Gene Symbol: PGM5
• Gene ID (NCBI): 5239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15124
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Phosphoglucomutase-like protein 5 (also known as aciculin) is an enzyme encoded by the PGM5 gene. Gene functional studies show that PGM5 is similar to PGM1 but lacks enzymatic activity. PGM5 is tightly associated with the actin cytoskeleton and has primarily been investigated as an adhesion protein. It also functions as a cytoskeletal component of cell-matrix and cell-cell contacts in muscle and non-muscle cells. (PMID: 35819729)