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  • Western Blot (WB) analysis of various lysates using PGM5 Recombinant antibody (82965-1-RR)

    WB analysis using 82965-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82965-1-RR (PGM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PGM5 Recombinant antibody (82965-1-RR)

    IHC staining of human stomach using 82965-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using PGM5 Recombinant antibody (82965-1-RR)

    IHC staining of human stomach using 82965-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of PC-3 cells using PGM5 Recombinant antibody (82965-1-RR)

    FC experiment of PC-3 using 82965-1-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human PGM5 (82965-1-RR, Clone:230206C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82965-1-RR

    Affinity and Kinetic Characterization of 82965-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82965-1-RR against Human PGM5 were performed. The affinity constant is 0.719 nM.

PGM5 Recombinant antibody

PGM5 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82965-1-RR
Clone No.230206C7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

PGMRP, Aciculin, 230206C7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82965-1-RR targets PGM5 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PGM5 fusion protein Ag34399 Predict reactive species
Full Name phosphoglucomutase 5
Calculated Molecular Weight 62 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberNM_021965
Gene Symbol PGM5
Gene ID (NCBI) 5239
RRIDAB_3670711
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15124
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphoglucomutase-like protein 5 (also known as aciculin) is an enzyme encoded by the PGM5 gene. Gene functional studies show that PGM5 is similar to PGM1 but lacks enzymatic activity. PGM5 is tightly associated with the actin cytoskeleton and has primarily been investigated as an adhesion protein. It also functions as a cytoskeletal component of cell-matrix and cell-cell contacts in muscle and non-muscle cells. (PMID: 35819729)

Protocols

Product Specific Protocols
WB protocol for PGM5 antibody 82965-1-RRDownload protocol
IHC protocol for PGM5 antibody 82965-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols