PGC1a Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PGC1a Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 66369-1-PBS

Synonyms

PPARGC1A, PGC 1v, PGC 1(alpha), PGC 1 alpha, Ligand effect modulator 6

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Validation Data Gallery

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  WB analysis using 66369-1-Ig (same clone as 66369-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66369-1-Ig (same clone as 66369-1-PBS)

  WB result of PGC1a antibody (66369-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and PGC1a knockout HeLa cells. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66369-1-Ig (same clone as 66369-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66369-1-Ig (same clone as 66369-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGC1a antibody (66369-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

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Product Information

66369-1-PBS targets PGC1a in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 70 kDa, 100 kDa
• GenBank Accession Number: NM_013261
• Gene Symbol: PGC1a
• Gene ID (NCBI): 10891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.