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PGC1a Monoclonal antibody
PGC1a Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig and More (6)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
396
CloneNo.
1C1B2
Cat no : 66369-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig skeletal muscle tissue, HSC-T6 cells, rat skeletal muscle tissue, mouse skeletal muscle tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 370 publications below |
| IHC | See 28 publications below |
| IF | See 51 publications below |
| IP | See 8 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
66369-1-Ig targets PGC1a in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, ducks |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | peroxisome proliferator-activated receptor gamma, coactivator 1 alpha |
| Calculated Molecular Weight | 91 kDa |
| Observed Molecular Weight | 70 kDa, 100 kDa |
| GenBank Accession Number | NM_013261 |
| Gene Symbol | PGC1a |
| Gene ID (NCBI) | 10891 |
| RRID | AB_2828002 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PGC1a antibody 66369-1-Ig | Download protocol |
| IF protocol for PGC1a antibody 66369-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice. | ||
J Hazard Mater Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers | ||
Nat Commun Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance. | ||
Nat Commun METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Michaela (Verified Customer) (11-24-2023) | Worked well in 15μg mouse brain tissue lysate in Western Blot. Band appears between 60-100 kDa
|
FH No (Verified Customer) (10-24-2023) | A good antibody to detect endogenous PGC1a.
 |
FH Mi (Verified Customer) (03-07-2023) | It doesn´t work well in human white and brown adipocyte cells, we got bands at unexpected size. Further KO validation may be needed.
|
FH Huai-Chin (Verified Customer) (07-11-2022) | There are several non-specific bands. At least there's a dominant band after adipogenesis and we can use this antibody at a fairly high dilution.
|
FH Mohammad (Verified Customer) (01-21-2020) | It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.
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