PFKP Monoclonal antibody, PBS Only

PFKP Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H4D4

Cat no : 68129-1-PBS

Synonyms

6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

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Validation Data Gallery

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  WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68129-1-Ig (same clone as 68129-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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Product Information

68129-1-PBS targets PFKP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PFKP fusion protein Ag25206
• Full Name: phosphofructokinase, platelet
• Calculated Molecular Weight: 784 aa, 86 kDa
• Observed Molecular Weight: 80-86 kDa
• GenBank Accession Number: BC029138
• Gene Symbol: PFKP
• Gene ID (NCBI): 5214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).