PFKP Monoclonal antibody

PFKP Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1H4D4

Cat no : 68129-1-Ig

Synonyms

6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

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Validation Data Gallery

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  WB analysis using 68129-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 68129-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68129-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

68129-1-Ig targets PFKP in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PFKP fusion protein Ag25206
• Full Name: phosphofructokinase, platelet
• Calculated Molecular Weight: 784 aa, 86 kDa
• Observed Molecular Weight: 80-86 kDa
• GenBank Accession Number: BC029138
• Gene Symbol: PFKP
• Gene ID (NCBI): 5214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

Publications

Species	Application	Title
human	WB	medRxiv; Depressed Proximal Glycolysis in Myocardium Of Human Heart Failure with Preserved Ejection Fraction; Authors - Navid Koleini
mouse	WB	Metabolism; Protein kinase N1 deficiency results in upregulation of cerebral energy metabolism and is highly protective in in vivo and in vitro stroke models; Authors - Stephanie Zur Nedden