PFKM Recombinant antibody, PBS Only (Detector)

PFKM Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241598B2

Cat no : 84281-5-PBS

Synonyms

PFK A, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type, 6-phosphofructokinase type A

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Validation Data Gallery

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  WB analysis using 84281-5-RR (same clone as 84281-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84281-5-RR (PFKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 84281-5-RR (same clone as 84281-5-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84281-5-RR (PFKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84281-5-RR (same clone as 84281-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84281-5-RR (PFKM antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84281-5-RR (same clone as 84281-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84281-5-RR (PFKM antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84281-5-RR (same clone as 84281-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PFKM antibody (84281-5-RR, Clone: 241598B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01204-3

  Cytometric bead array standard curve of MP01204-3, PFKM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84281-4-PBS. Detection antibody: 84281-5-PBS. Standard: Ag30840. Range: 0.156-20 ng/mL.

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Product Information

84281-5-PBS targets PFKM as part of a matched antibody pair:

MP01204-3: 84281-4-PBS capture and 84281-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PFKM fusion protein Ag30840
• Full Name: phosphofructokinase, muscle
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC021203
• Gene Symbol: PFKM
• Gene ID (NCBI): 5213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM cause glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular weights of 85, 82, and 93 kDa.