PFKL Monoclonal antibody, PBS Only

PFKL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2G2

Cat no : 68385-1-PBS

Synonyms

PFK B, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, liver type, 6-phosphofructokinase type B

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Validation Data Gallery

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  WB analysis using 68385-1-Ig (same clone as 68385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68385-1-Ig (PFKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IF Staining of HuH-7 using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  IF Staining of HuH-7 using 68385-1-Ig (same clone as 68385-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 68385-1-Ig (same clone as 68385-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug PFKL Monoclonal antibody (68385-1-Ig, Clone:1H2G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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Product Information

68385-1-PBS targets PFKL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PFKL fusion protein Ag8250
• Full Name: phosphofructokinase, liver
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC008964
• Gene Symbol: PFKL
• Gene ID (NCBI): 5211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PFKL (6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings, PFK exists as a system of three subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs (PMID: 2139864). It has 2 isoforms produced by alternative splicing.