Various lysates were subjected to SDS PAGE followed by western blot with 68385-1-Ig (PFKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68385-1-Ig (PFKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
IHC staining of human lung cancer using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HuH-7 using 68385-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HuH-7 using 68385-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC experiment of HEK-293 using 68385-1-Ig
1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug PFKL Monoclonal antibody (68385-1-Ig, Clone:1H2G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug PFKL Monoclonal antibody (68385-1-Ig, Clone:1H2G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human lung cancer tissue, human liver tissue, human ovary tumor tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HuH-7 cells
Positive FC (Intra) detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PFKL (6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings, PFK exists as a system of three subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs (PMID: 2139864). It has 2 isoforms produced by alternative splicing.
Identification of hub glycolysis-related genes in acute myocardial infarction and their correlation with immune infiltration using bioinformatics analysis
Various lysates were subjected to SDS PAGE followed by western blot with 68385-1-Ig (PFKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
IHC Figures
IHC staining of human lung cancer using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 68385-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HuH-7 using 68385-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HuH-7 using 68385-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC (INTRA) Figures
FC experiment of HEK-293 using 68385-1-Ig
1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug PFKL Monoclonal antibody (68385-1-Ig, Clone:1H2G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PFKL Monoclonal antibody
Catalog Number
68385-1-Ig
Citations
2
Dilutions
WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat, rabbit, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
