PEX5 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PEX5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C2C3

Cat no : 66309-1-PBS

Synonyms

Cell and organelle markers, Peroxin 5, Peroxisome Marker, Peroxisome receptor 1, PEX5, PTS1 BP, PTS1 receptor, PTS1R, PXR1

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Validation Data Gallery

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  WB analysis of HEK-293 using 66309-1-Ig (same clone as 66309-1-PBS)

  WB result of PEX5 antibody (66309-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX5 transfected HEK-293 cells. This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66309-1-Ig (same clone as 66309-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66309-1-Ig (same clone as 66309-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66309-1-Ig (same clone as 66309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66309-1-Ig (same clone as 66309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66309-1-Ig (same clone as 66309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66309-1-Ig (same clone as 66309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

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Product Information

66309-1-PBS targets PEX5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PEX5 fusion protein Ag3268
• Full Name: peroxisomal biogenesis factor 5
• Calculated Molecular Weight: 631 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC010621
• Gene Symbol: PEX5
• Gene ID (NCBI): 5830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder.