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PEX5 Monoclonal antibody

PEX5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C2C3

Cat no : 66309-1-Ig

Synonyms

Peroxin 5, Peroxisome Marker, Peroxisome receptor 1, PEX5, PTS1 BP, PTS1 receptor, PTS1R, PXR1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of HEK-293 using 66309-1-Ig

    WB result of PEX5 antibody (66309-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX5 transfected HEK-293 cells.

  • Western Blot (WB) analysis of pig brain tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of pig brain using 66309-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of fetal human brain using 66309-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human liver using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human liver using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human kidney using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human kidney using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inpig brain tissue, HEK-293 cells, fetal human brain tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66309-1-Ig targets PEX5 in WB, IHC, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEX5 fusion protein Ag3268
Full Name peroxisomal biogenesis factor 5
Calculated Molecular Weight 631 aa, 70 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC010621
Gene Symbol PEX5
Gene ID (NCBI) 5830
RRIDAB_2881691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder.

Protocols

Product Specific Protocols
WB protocol for PEX5 antibody 66309-1-IgDownload protocol
IHC protocol for PEX5 antibody 66309-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

BNIP3L/NIX regulates both mitophagy and pexophagy

Authors - Léa P Wilhelm
View Article