PEX14 (human specific) Recombinant antibody

PEX14 (human specific) Recombinant Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

6K5

Cat no : 80196-1-RR

Synonyms

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, PEX14 (human specific), Pex14p, PTS1 receptor docking protein



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80196-1-RR targets PEX14 (human specific) in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEX14 (human specific) fusion protein Ag0932
Full Name peroxisomal biogenesis factor 14
Calculated Molecular Weight 41 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC006327
Gene Symbol PEX14
Gene ID (NCBI) 5195
RRIDAB_2918874
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

This antibody is specific to human PEX14. It can't detect mouse or rat PEX14.

Protocols

Product Specific Protocols
WB protocol for PEX14 (human specific) antibody 80196-1-RRDownload protocol
IHC protocol for PEX14 (human specific) antibody 80196-1-RRDownload protocol
IF protocol for PEX14 (human specific) antibody 80196-1-RRDownload protocol
FC protocol for PEX14 (human specific) antibody 80196-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols