PEX14 (human specific) Recombinant antibody

PEX14 (human specific) Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

6K5

Cat no : 80196-1-RR

Synonyms

PEX14, PEX, Peroxin-14, Peroxin 14, NAPP2

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Validation Data Gallery

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  WB analysis using 80196-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 80196-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 80196-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 80196-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 80196-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 80196-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 (human specific) antibody (80196-1-RR, Clone: 6K5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 80196-1-RR

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (human specific) (80196-1-RR, Clone:6K5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 80196-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80196-1-RR against PEX14 (human specific) were performed. The affinity constant is 0.613 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80196-1-RR targets PEX14 (human specific) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PEX14 (human specific) fusion protein Ag0932
• Full Name: peroxisomal biogenesis factor 14
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006327
• Gene Symbol: PEX14
• Gene ID (NCBI): 5195
• RRID: AB_2918874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

This antibody is specific to human PEX14. It can't detect mouse or rat PEX14.