PEX14 (human specific) Recombinant antibody, PBS Only

PEX14 (human specific) Recombinant Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6K5

Cat no : 80196-1-PBS

Synonyms

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, PEX14 (human specific), Pex14p, PTS1 receptor docking protein

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Validation Data Gallery

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  WB analysis using 80196-1-RR (same clone as 80196-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80196-1-RR (same clone as 80196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80196-1-RR (same clone as 80196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 80196-1-RR (same clone as 80196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 80196-1-RR (same clone as 80196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80196-1-RR (same clone as 80196-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX14 antibody (80196-1-RR, Clone: 6K5 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 80196-1-RR (same clone as 80196-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (human specific) (80196-1-RR, Clone:6K5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80196-1-PBS targets PEX14 (human specific) in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PEX14 (human specific) fusion protein Ag0932
• Full Name: peroxisomal biogenesis factor 14
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006327
• Gene Symbol: PEX14
• Gene ID (NCBI): 5195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

This antibody is specific to human PEX14. It can't detect mouse or rat PEX14.