CoraLite® Plus 488-conjugated PER2 (isoform 1) Monoclonal antibody

PER2 (isoform 1) Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E6C3

Cat no : CL488-67513

Synonyms

PER2, PER 2, hPER2, FASPS, 2E6C3

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67513

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PER2 (isoform 1) antibody (CL488-67513, Clone: 2E6C3 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-67513

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Per2 (Isoform 1) Monoclonal Antibody (CL488-67513, Clone:2E6C3)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67513 targets PER2 (isoform 1) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PER2 (isoform 1) fusion protein Ag29489
• Full Name: period homolog 2 (Drosophila)
• Calculated Molecular Weight: 137 kDa
• Observed Molecular Weight: 150~160 kDa
• GenBank Accession Number: NM_022817
• Gene Symbol: PER2
• Gene ID (NCBI): 8864
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).