PEG3 Recombinant antibody

PEG3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241234C5

Cat no : 84030-5-RR

Synonyms

Zinc finger and SCAN domain-containing protein 24, PW1, Paternally-expressed gene 3 protein, paternally expressed 3, KIAA0287

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Validation Data Gallery

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  WB analysis of TT using 84030-5-RR

  TT cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 84030-5-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84030-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84030-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84030-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PEG3 antibody (84030-5-RR, Clone: 241234C5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 84030-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PEG3 Recombinant antibody (84030-5-RR, Clone:241234C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84030-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84030-5-RR against Human PEG3 were performed. The affinity constant is 52.3 pM.

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Tested Applications

• Positive WB detected in: TT cells, HepG2 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84030-5-RR is PEG3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PEG3 fusion protein Ag20132
• Full Name: paternally expressed 3
• Calculated Molecular Weight: 1588 aa, 181 kDa
• GenBank Accession Number: BC150272
• Gene Symbol: PEG3
• Gene ID (NCBI): 5178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).