PEG3 Recombinant antibody, PBS Only

PEG3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241234C5

Cat no : 84030-5-PBS

Synonyms

Zinc finger and SCAN domain-containing protein 24, PW1, Paternally-expressed gene 3 protein, paternally expressed 3, KIAA0287

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Validation Data Gallery

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  WB analysis of TT using 84030-5-RR (same clone as 84030-5-PBS)

  TT cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 84030-5-RR (same clone as 84030-5-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84030-5-RR (same clone as 84030-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84030-5-RR (same clone as 84030-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84030-5-RR (same clone as 84030-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PEG3 antibody (84030-5-RR, Clone: 241234C5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 84030-5-RR (same clone as 84030-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PEG3 Recombinant antibody (84030-5-RR, Clone:241234C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84030-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84030-5-RR against Human PEG3 were performed. The affinity constant is 52.3 pM.

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Product Information

The immunogen of 84030-5-PBS is PEG3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PEG3 fusion protein Ag20132
• Full Name: paternally expressed 3
• Calculated Molecular Weight: 1588 aa, 181 kDa
• GenBank Accession Number: BC150272
• Gene Symbol: PEG3
• Gene ID (NCBI): 5178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).