Tested Applications
Positive WB detected in | human heart tissue, human platelets |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67651-1-Ig targets PDLIM5 in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Immunogen | PDLIM5 fusion protein Ag25059 Predict reactive species |
Full Name | PDZ and LIM domain 5 |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 63-69 kDa |
GenBank Accession Number | BC008741 |
Gene Symbol | PDLIM5 |
Gene ID (NCBI) | 10611 |
RRID | AB_2882850 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Euglobulin precipitation |
UNIPROT ID | Q96HC4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PDLIM5 antibody 67651-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |