PDLIM5 Monoclonal antibody

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  WB analysis using 67651-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67651-1-Ig (PDLIM5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PDLIM5 Monoclonal Antibody for WB, ELISA

Cat No. 67651-1-Ig

Clone No.1H9C9

Host / Isotype

Mouse / IgM

Reactivity

Human

Applications

WB, ELISA

Synonyms

ENH, ENH1, Enigma homolog, L9, LIM, PDLIM5, PDZ and LIM domain 5, PDZ and LIM domain protein 5

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Tested Applications

• Positive WB detected in: human heart tissue, human platelets

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67651-1-Ig targets PDLIM5 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDLIM5 fusion protein Ag25059
• Full Name: PDZ and LIM domain 5
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 63-69 kDa
• GenBank Accession Number: BC008741
• Gene Symbol: PDLIM5
• Gene ID (NCBI): 10611
• RRID: AB_2882850
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Euglobulin precipitation
• UNIPROT ID: Q96HC4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression.