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PDLIM5 Monoclonal antibody, PBS Only

PDLIM5 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9C9

Cat no : 67651-1-PBS

Synonyms

ENH, ENH1, Enigma homolog, L9, LIM, PDLIM5, PDZ and LIM domain 5, PDZ and LIM domain protein 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDLIM5 Monoclonal antibody (67651-1-Ig)

    WB analysis using 67651-1-Ig (same clone as 67651-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67651-1-Ig (PDLIM5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67651-1-PBS in a different storage buffer formulation.

Product Information

67651-1-PBS targets PDLIM5 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen PDLIM5 fusion protein Ag25059
Full Name PDZ and LIM domain 5
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 63-69 kDa
GenBank Accession NumberBC008741
Gene Symbol PDLIM5
Gene ID (NCBI) 10611
Conjugate Unconjugated
Form Liquid
Purification MethodEuglobulin precipitation
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression.