PDLIM4 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 84117-1-RR (same clone as 84117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84117-1-RR (PDLIM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84117-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84117-1-RR (same clone as 84117-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84117-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84117-1-RR (same clone as 84117-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84117-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01021-1

  Cytometric bead array standard curve of MP01021-1, PDLIM4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84117-4-PBS. Detection antibody: 84117-1-PBS. Standard: Ag13424. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01021-2

  Cytometric bead array standard curve of MP01021-2, PDLIM4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84117-2-PBS. Detection antibody: 84117-1-PBS. Standard: Ag13424. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01021-3

  Cytometric bead array standard curve of MP01021-3, PDLIM4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84117-3-PBS. Detection antibody: 84117-1-PBS. Standard:Ag13424. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84117-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84117-1-RR against Human PDLIM4 were performed. The affinity constant is 2.43 nM.

PDLIM4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 84117-1-PBS

Clone No.241401B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

RIL, Reversion-induced LIM protein, PDZ and LIM domain 4, 241401B3

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Product Information

84117-1-PBS targets PDLIM4 as part of a matched antibody pair:

MP01021-1: 84117-4-PBS capture and 84117-1-PBS detection (validated in Cytometric bead array)

MP01021-2: 84117-2-PBS capture and 84117-1-PBS detection (validated in Cytometric bead array)

MP01021-3: 84117-3-PBS capture and 84117-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDLIM4 fusion protein Ag13424
• Full Name: PDZ and LIM domain 4
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC016765
• Gene Symbol: PDLIM4
• Gene ID (NCBI): 8572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50479
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID:12908099).