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  • Western Blot (WB) analysis of various lysates using PDLIM4 Recombinant antibody (84117-1-RR)

    WB analysis using 84117-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84117-1-RR (PDLIM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PDLIM4 Recombinant antibody (84117-1-RR)

    IF Staining of U2OS using 84117-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PDLIM4 Recombinant antibody (84117-1-RR)

    IF Staining of U2OS using 84117-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84117-1-RR

    Affinity and Kinetic Characterization of 84117-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84117-1-RR against Human PDLIM4 were performed. The affinity constant is 2.43 nM.

PDLIM4 Recombinant antibody

PDLIM4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84117-1-RR
Clone No.241401B3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RIL, Reversion-induced LIM protein, PDZ and LIM domain 4, 241401B3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inA431 cells, K-562 cells, PC-3 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84117-1-RR targets PDLIM4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDLIM4 fusion protein Ag13424 Predict reactive species
Full Name PDZ and LIM domain 4
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC016765
Gene Symbol PDLIM4
Gene ID (NCBI) 8572
RRIDAB_3671681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50479
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID:12908099).

Protocols

Product Specific Protocols
WB protocol for PDLIM4 antibody 84117-1-RRDownload protocol
IF protocol for PDLIM4 antibody 84117-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols