PDK1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PDK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18262-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18262-1-AP (PDK1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18262-1-AP (PDK1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 18262-1-AP (same clone as 18262-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDK1 antibody (18262-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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Product Information

18262-1-PBS targets PDK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDK1 fusion protein Ag13121
• Full Name: pyruvate dehydrogenase kinase, isozyme 1
• Calculated Molecular Weight: 436 aa, 49 kDa
• Observed Molecular Weight: 46-55 kDa
• GenBank Accession Number: BC039158
• Gene Symbol: PDK1
• Gene ID (NCBI): 5163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is specific to PDK1.