CoraLite®594-conjugated PDIA6 Monoclonal antibody

PDIA6 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G2D4

Cat no : CL594-66669

Synonyms

Protein disulfide isomerase P5, Protein disulfide isomerase A6, P5, ERp5, ER protein 5

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66669

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66669 (PDIA6 antibody) at dilution of 1:100.

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  FC experiment of HepG2 using CL594-66669

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PDIA6 (CL594-66669, Clone:1G2D4) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66669 targets PDIA6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDIA6 fusion protein Ag27608
• Full Name: protein disulfide isomerase family A, member 6
• Calculated Molecular Weight: 48 kDa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC001312
• Gene Symbol: PDIA6
• Gene ID (NCBI): 10130
• RRID: AB_2883612
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.