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ERp72 Polyclonal antibody

ERp72 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 14712-1-AP


Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERP72, PDIA4, Protein disulfide isomerase A4

Tested Applications

Positive WB detected inmouse brain tissue, L02 cells, HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, PC-3 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14712-1-AP targets ERp72 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERp72 fusion protein Ag6431
Full Name protein disulfide isomerase family A, member 4
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC000425
Gene Symbol PDIA4
Gene ID (NCBI) 9601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.


Product Specific Protocols
WB protocol for ERp72 antibody 14712-1-APDownload protocol
IHC protocol for ERp72 antibody 14712-1-APDownload protocol
IF protocol for ERp72 antibody 14712-1-APDownload protocol
IP protocol for ERp72 antibody 14712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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